Step 1: Add Microbe-Lift/Special Blend.Speeds the removal of all organic waste matter in aquariums by adding a select group of microbes that increases the rate of organic breakdown improves filter performance and removes toxic nitrate.Step 2: Add Microbe-Lift Nite-Out IIProvides necessary nitrifying bacteria (Nitrosomonas sp. Nitrobacter sp. Nitrospira.) required for the biological nitrification process and assures the continued removal of toxic ammonia and nitrate!